EPU Data Collection

A hands-on microscope operation workshop for EPU SPA Data Collection.  Prerequisite: SerialEM Introductory Training Workshop, SerialEM Data Collection Workshop or equivalent.

Clock

On-site

Clock

5 Days

User

6-8
Trainees

Sun

Morning
Practical

Afternoon

Afternoon
Practical

Afternoon

No Cost

Dates:  TBD 2025

Travel Costs: Covered by applicant

Accommodations: Covered by applicant (discounts at local hotels are available through PNCC)

Daily lunches and refreshments: Provided by PNCC

Location: RLSB

Who: PNCC Awarded General Access Proposal members only


The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five-day on-site training workshop for 6-8 trainees on EPU data collection at PNCC TBD 2025. This course is focused on training users to independently operate the microscope and collect data for single particle studies. This is an advanced course where students are required to have basic understanding of the Microscope UI. Students are required to take the prerequisite course SerialEM Screening workshop or SerialEM Data Collection workshop prior to applying.


APPLY HERE


NOTE: Workshop dates will be determined when enough suitable applicants have applied. To maximize impact, PNCC prefers to train one lab member per group with the intention that member further trains others in their group. Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Application closes (TBD 2025). 

 

Agenda

Each day includes a combination of presentations, demonstrations, and hands-on practice. Workshop runs from 9am to 5pm (Pacific) each day.

Day 1

  • Welcome & Workshop Overview
  • Introduction to the EPU Data Collection Interface and Workflow
  • Hands-On Learning: Beam Alignments

Day 2

  • Introduction to the Selectris energy filter
  • Introduction to Sherpa
  • Hands-On Learning: Screening with EPU

Day 3

  • Introduction to Target Selection and Collection Methods
  • Hands-On Learning: Full Workflow
  • Group Dinner

Day 4

  • Review of Workflow
  • Introduction to Multigrid EPU
  • Common Troubleshooting

Day 5

  • EPU Data Collection Merit Badge Evaluation
  • Data Collection on Mystery Sample
  • Workshop Wrap-Up and Next Steps