EPU Data Collection

A hands-on microscope operation workshop for EPU SPA Data Collection.  Prerequisite: SerialEM Introductory Training Workshop or SerialEM Data Collection Workshop.

Dates:  TBD 2025

Lodging: Room and Board provided

Accommodations: University Place Hotel or Hyatt House

Location: RLSB

Who: PNCC Awarded General Access Proposal members only

The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five-day on-site training workshop for 4-6 trainees on EPU data collection at PNCC TBD 2025. This course is focused on training users to independently operate the microscope and collect data for single particle studies. This is an advanced course where students are required to have basic understanding of the Microscope UI. Students are required to take the prerequisite course SerialEM Screening workshop or SerialEM Data Collection workshop prior to applying.

APPLY HERE


NOTE: Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Application closes TBD 2025. 

Agenda

Each day includes a combination of presentations, demonstrations, and hands-on practice. Workshop runs from 9am to 5pm (Pacific) each day.

Day 1

• Welcome & Workshop Overview
• Introduction to the EPU Data Collection Interface and Workflow
• Hands-On Learning: Beam Alignments

Day 2

• Introduction to the Selectris energy filter
• Introduction to Sherpa
• Hands-On Learning: Screening with EPU

Day 3

• Introduction to Target Selection and Collection Methods
• Hands-On Learning: Full Workflow
• Group Dinner

Day 4

• Review of Workflow
• Introduction to Multigrid EPU
• Common Troubleshooting

Day 5

• EPU Data Collection Merit Badge Evaluation
• Data Collection on Mystery Sample
• Workshop Wrap-Up and Next Steps