Publications listed represent all or part of the research that was carried out using PNCC resources.

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PNCC Publications


  1. Wiryanman, T. & Toor, N. (2021). Cryo-EM structure of a thermostable bacterial nanocompartment. IUCrJ.
  2. Noviello, C. M., Gharpure, A., Mukhtasimova, N., Cubuco, R., Baxter, L., Borek, D., Sine, S. M., & Hibbs, R. E. (2021). Structure and gating mechanism of the α7 nicotinic acetylcholine receptor. Cell.
  3. Chen, S., Lee, L., Naila, T., Fishbain, S., Wang, A., Tomkinson, A. E., Lees-Miller, S. P., & He, Y. (2021). Structural basis of long-range to stort-range synaptic transition in NHEJ. Nature.
  4. Osinski, A., Black, M. H., Pawłowski, K., Chen, Z., Li, Y., & Tagliabracci, V. S. (2021). Structural and mechanistic basis for protein glutamylation by the kinase fold. Preprint on bioRxiv.
  5. Abdella, R., Talyzina, A., Chen, S., Inouye, C. J., Tjian, R., & He, Y. (2021). Structural of the human Mediator-bound transcription preinitiation complex. Science.
  6. Grau, D., Zhang, Y., Lee, C-H., Valencia-Sanchez, M., Zhang, J., Wang, M., Holder, M., Svetlov, V., Tan, D., Nudler, E., Reinberg, D., Walz, T., Armache, K-J. (2021). Structures of the monomeric and dimeric PRC2:EZH2 reveal flexible modules involved in chromatin compaction. Nature Communications.
  7. Liu, C., Reese, R., Vu, S., Rouge, L., Shields, S. D., Kakiuchi-Kiyota, S., Chen, H., Johnson, K., Shi, Y. P., Chernov-Rogan, T., Greiner, D. M. Z., Kohli, P. B., Hackos, D., Brillantes, B., Tam, C., Li, T., Wang, J., Safina, B., Magnuson, S., Volgraf, M., Payandeh, J., Zheng, J., Rohou, A., & Chen, J. (2021). A Non-covalent Ligand Reverals Biased Agonism of the TRPA1 Ion Channel. Neuron.
  8. Hollingsworth, L. R., David, L., Li, Y., Griswold, A. R., Sharif, H., Fontana, P., Fu, T., Ruan, J., Bachovchin, D. A., & Wu, H. (2021). Novel mode of filament formation in UPA-promoted CARD8 and NLRP1 Inflammasomes. Nature Communications.
  9. Sharif, H., Hollingsworth, L. R., Griswold, A. R., Hsiao, J. C., Wang, Q., Bachovchin, D. A., & Wu, H. (2021). Structural mechanism of CARD8 regulation by DPP9. Preprint on bioRxiv.
  10. Shen, B. W., Quispe, J. D., Luyten, Y., McGough, B. E., Morgan, R. D., & Stoddard, B. L. (2021). Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM. Preprint on bioRxiv.
  11. Sweet, M. E., Larsen, C., Zhang, X., Schlame, M., Pedersen, B. P., & Stokes, D. L. (2021). Structural basis for Potassium transport by KdpFABC. Preprint on bioRxiv.
  12. Travis, B. A., Ramsey, K. M.,Prezioso, S. M., Tallo, T., Wandzilak, J. M., Hsu, A., Borgnia, M., Bartesaghi, A., Dove, S. L., Brennan, R. G., & Schumacher, M. A. (2021). Structural Basis for Virulence Activation of Francisella tularensis. Molecular Cell.



  1. Yoder, N., Jalali-Yazdi, F., Noreng, S., Houser, A., Baconguis, I., & Gouaux, E. (2020). Light-coupled cryo-plunger for time-resolved cryo-EM. Journal of Structural Biology.
  2. Bonilla, S., Sherlock, M. E., MacFadden, A., & Kieft, J. S. (2020). A structured viral RNA uses molecular mimicry and conformational dynamics to coordinate multiple functions. Preprint on bioRxiv.
  3. Coleman, J. A., Navratna, V., Antermite, D., Yang, D., Bull, J. A., & Gouaux, E. (2020). Chemical and structural investigation of the paroxetine-human serotonin transporter complex. eLife.
  4. Coudray, N., Isom, G. L., MacRae, M. R., Saiduddin, M. N., Bhabha, G., & Ekiert, D. C. (2020). Structure of bacterial phospholipid transporter MlaFEDB with substrate bound. Preprint on bioRxiv.
  5. Eygeris, Y., Patel, S., Jozic, A., & Sahay, G. (2020). Deconvoluting Lipid Nanoparticle Structure for Messenger RNA Delivery. ACS Publications.
  6. Flores, J. A., Haddad, B. G., Dolan, K. A., Myers, J. B., Yoshioka, C. C., Copperman, J., Zuckerman, D. M., & Reichow, S. L. (2020). Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å. Nature Communications.
  7. Herrera, M., Kim, J., Eygeris, Y., Jozic, A., & Sahay, G. (2020). Illuminating endosomal escape of polymorphic lipid nanoparticles that boost mRNA delivery. Preprint on bioRxiv.
  8. Hollingsworth, L. R., Sharif, H., Griswold, A. R., Fontana, P., Mintseris, J., Dagbay, K. B., Paulo, J. A., Gygi, S. P., Bachovchin, D. A., & Wu, H. (2020). DPP9 directly sequesters the NLRP1 C-terminus to repress inflammasome activation. Preprint on bioRxiv.
  9. Jette, C. A., Barnes, C. O., Kirk, S. M., Melillo, B., Smith, A. B., & Bjorkman, P. J. (2020). Cryo-EM Structures of HIV-1 trimer bound to CD4-mimetics M48U1 and BNM-III-170 adopt a CD4-bound open conformation. Preprint on bioRxiv.
  10. Kato, K., Ahmad, S., Zhu, Z., Young, J. M., Mu, X., Park, S., Malik, H. S., & Hur, S. (2020). Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases. Molecular Cell.
  11. Kim, J. J., Gharpure, A., Teng, J., Zhuang, Y., Howard, R. J., Zhu, S., Noviello, C., Walsh Jr, R. M., Lindahl, E., & Hibbs, R. E. (2020). Shared structural mechanisms of general anaesthetics and benzodiazepines. Nature.
  12. Lopez-Redondo, M., Fan, S., Koide, A., Koide, S., Beckstein, O., & Stokes, D. L. (2020). Zinc Dependent Conformational Changes in the Cation Diffusion Facilitator YiiP from S. oneidensis. Preprint on bioRxiv.
  13. Novikova, I. V., Zhou, M., Du, C., Parra, M., Kim, D. N., VanAernum, Z. L., Shaw, J. B., Hellmann, H., Wysocki, V. H., & Evans, J. E. (2020). Tunable hetero-assembly of a plant pseudoenzyme-enzyme complex. Preprint on bioRxiv.
  14. Rahman, M. M., Teng, J., Worrell, B. T., Noviello, C. M., Lee, M., Karlin, A., Stowell, M. H. B., & Hibbs, R. E. (2020). Structure of the Native Muscle-type Nicotinic Receptor and Inhibition by Snake Venom Toxins. Neuron.
  15. Santosh, V., Musayev, F., Jaiswal, R., Zárate-Pérez, F., Vandewinkel, B., Dierckx, C., Endicott, M., Sharifi, K., Dryden, K., Henckaerts, E., & Escalante, C. R. (2020). The Cryo-EM structure of AAV2 Rep68 in complex with ssDNA reveals a malleable AAA+ machine that can switch between oligomeric states. Nucleic Acids Research.
  16. Silveria, M. A., Large, E. E., Zane, G. M., White, T. A., & Chapman, M. S. (2020). The Structure of an AAV5-AAVR Complex at 2.5 Å Resolution: Implications for Cellular Entry and Immune Neutralization of AAV Gene Therapy Vectors. Viruses.
  17. Wang, Z., Rahkola, J., Redzic, J. S., Chi, Y-C., Tran, N., Holyoak, T., & Zheng, H. (2020). Mechanism and inhibition of Streptococcus pneumoniae IgA1 protease. Nature Communications.
  18. Sobti, M., Walshe, J. L., Ishmukhametov, R., & Stewart, A. G. (2020). Visualizing movements in E. coli F1Fo ATP synthase indicates how the F1 and Fo motors are coupled. Nature Communications.
  19. Sobti, M., Walshe, J. L., Zeng, Y. C., Ishmukhametov, R., & Stewart, A. G. (2020). Subunit epsilon of E. coli F1Fo ATP synthase attenuates enzyme activity by modulating central stalk flexibility. Preprint on bioRxiv.
  20. Unchwaniwala, N., Zhan, H., Pennington, J., Horswill, M., den Boon, J. A., & Ahlquist, P. (2020). Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication. PNAS.
  21. Wang, C., Molodtsov, V., Firlar, E., Kaelber, J., Blaha, G., Su, M., & Ebright, R. H. (2020). Structural basis of transcription-translation coupling. Science.
  22. Yang, X., Wang, Q., & Cao, E. (2020). Structure of the human cation-chloride cotransporter NKCC1 determined by single-particle electron cryo-microscopy. Nature Communications.
  23. Yang, J. E., Larson, M. R., Sibert, B. S., Shrum, S., & Wright, E. R. (2020). CorRelator: An interactive and flexible toolkit for high-precision cryo-correlative light and electron microscopy. bioRxiv, 2020.08.06.240481. Preprint on bioRxiv.
  24. Yoder, N., & Gouaux, E. (2020). Conserved His-Gly motif of acid-sensing ion channels resides in a reentrant ‘loop’ implicated in gating and ion selectivity. eLife.
  25. Zhao, Y., Shen, J., Wang, Q., Zhou, M., & Cao, E. (2020). Inhibitory and Transport Mechanisms of the Human Cation-Chloride Cotransport KCC1. Preprint on bioRxiv.



  1. Novikova, I., Zhou, M., Shaw, J., Hellmann, H., & Evans, J. E. (2019). Dual Native MS and Cryo-EM Approach to Resolve Heteromeric Protein Assemblies and Subunit Stoichiometry. Cambridge University Press.
  2. Patel, A. B., Moore, C. M., Greber, B. J., Luo, J., Ranish, J., & Nogales, E. (2019). Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement. eLife.
  3. Yoshioka, C. (2019). Introducing the Pacific Northwest Center for Cryo-EM. Cambridge University Press.