Major instrumentation available at PNCC include 1 FEI Talos Arctica and 4 FEI Titan Krios instruments for training, sample screening and high-resolution data acquisition. Gatan K2 Direct Electron Detectors and sample autoloaders are installed on all microscopes for optimal resolution and throughput. One Titan Krios incorporates a FEI phase plate for high-contrast applications in both cryo-electron tomography and single particle imaging. In addition, PNCC maintains a wide array of equipment sample characterization and preparation using single particle and tomography methods.
Arctica Microscopes

Arctica

Science Applications
Screening (limited)
Single Particle
Equipped With
Autoloader
K3 DED
Performance
2Å Apoferritin or better
Future Upgrade
Fringe Free (TBD 2022)
Data Collection Rate (per 24 hrs)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-1 Microscope

Krios-1

Science Applications
Screening (limited)
Single Particle
Tomography
Equipped With
Autoloader
Falcon 3 DED
K3 DED
Fringe Free
Performance
1.6Å Apoferritin or better
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-2 Microscope

Krios-2

Science Applications
Screening (limited)
Single Particle
Tomography
Equipped With
Autoloader
Falcon 3 DED
K3 DED
BioContinuum K3
Fringe Free
Performance
1.6Å Apoferritin or better
Future Upgrade
E-CFEG (TBD)
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-3 Microscope

Krios-3

Science Applications
Screening (limited)
Single Particle
Tomography
Equipped With
Autoloader
Falcon 3 DED
Phase Plate
Bioquantum K3
Performance
1.6Å Apoferritin or better
Future Upgrade
Fringe Free (TBD 2022)
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-4 Microscope

Krios-4

Science Applications
Screening (limited)
Single Particle
Tomography
Equipped With
Autoloader
Falcon 3 DED
Phase Plate
Bioquantum K3
Fringe Free
Performance
1.6Å Apoferritin or better
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)