Instruments
Major instrumentation available at PNCC will include one TFS Talos Arctica and four TFS Titan Krios instruments for training, sample screening, and high-resolution data acquisition. Falcon II or III and Gatan K2 or K3 Direct Electron Detectors (DEDs) and sample autoloaders are installed on all microscopes for optimal resolution and throughput. Two Titan Krios' incorporate a phase plate and Bioquantum energy filter for high-contrast applications in both cryo-electron tomography and single-particle imaging. In addition, PNCC maintains a wide array of equipment for sample characterization and preparation using single-particle and tomography methods. The TFS Talos Arctica became available for use starting November 1, 2018 and the other microscopes are all operational as of September 2019. 

Microscope

Science
Applications

Currently
Equipped With

Performance
Test Falcon 2/3 
Detector 

Performance
Test K2/K3 
Detector 

User
Results

Example
Dataset *

PNCC Arctica

CALENDAR

Screening,
Single Particle

Autoloader,
K2 DED

N/A

2-0.5Å (K2)
2.3Å Best (K2)

± 4Å: 8+

± 6Å: 8+

Apoferritin

OHSU MMC Krios 
(limited time)

Screening (Limited),
Single Particle,
Tomography

Autoloader,
Phase Plate,
Falcon 2 DED,
Bioquantum K3

2-0.5Å
1.6Å Best

2-0.5Å (K3)
2.0Å Best (K3)

± 3Å: 6+

± 4Å: 10+

Apoferritin

PNCC Krios #1

Screening (Limited),
Single Particle,
Tomography

Autoloader,
Falcon 3 DED 

2-0.5Å 
2Å Best

K3 Expected 10/2019

TBA

Apoferritin

PNCC Krios #2

Screening (Limited),
Single Particle,
Tomography

Autoloader,
Falcon 3 DED

2-0.5Å
In Progress

K3 Expected 06/2020

TBA

Apoferritin

PNCC Krios #3

Screening (Limited),
Single Particle,
Tomography

Autoloader,
Phase Plate,
Falcon 3 DED,
Bioquantum K3

TBA

K3 Expected 09/2019

TBA

Apoferritin

        *To access the example data, you must register for an EMSL User Account as described on the ACCESS page.
            To download, 1) login to the EMSL User Portal, 2) click on the above links, 3) select the boxes next to the dataset of interest,
            4) click to generate a cart, and 5) once cart is generated click the new link to download data.  

 

  

TFS Talos Arctica
TALOS ARCTICA: PNCC’S MAIN SAMPLE SCREENING AND INITIAL DATA COLLECTION PLATFORM
The TFS Talos Arctica is an automated high-throughput transmission electron microscope (TEM) platform focused on single-particle analysis. The constant-power, C-Twin lens delivers outstanding optical performance to allow optimal balance of contrast and resolution. The Arctica is an ideal instrument for screening cryo samples, for exploratory data collection, and for moderately advanced training.  It is also a very capable high-resolution data collection instrument, and is capable of achieving single-particle reconstructions of < 3Å. 
TITAN KRIOS: PNCC’S HIGH-RESOLUTION DATA ACQUISITION PLATFORM
The TFS Titan Krios cryo-transmission electron microscope is a state-of-the-art 300kV instrument optimized for both high-resolution 3D imaging of proteins and macromolecular assemblies, and high-resolution cellular tomography. Its revolutionary design and stability permit a full range of semi-automated applications, including 2D electron crystallography, single-particle analysis, cryo-electron microscopy, and cellular tomography of frozen hydrated samples.  The use of 300kV energy, addition of a third condenser lens, and increased optical stability make this the ideal instrument for collecting very large datasets at highest possible resolutions. 
TFS Titan Krios cryo-Transmission Electron Microscope