Major instrumentation available at PNCC will include one TFS Talos Arctica and three TFS Titan Krios instruments for training, sample screening, and high-resolution data acquisition. Falcon III and Gatan K2, or K3 Direct Electron Detectors and sample autoloaders will be installed on all microscopes for optimal resolution and throughput. One Titan Krios will incorporate a phase plate and/or energy filter for high-contrast applications in both cryo-electron tomography and single-particle imaging. In addition, PNCC maintains a wide array of equipment for sample characterization and preparation using single-particle and tomography methods. The TFS Talos Arctica became available for use starting November 1, 2018 and the other three microscopes will be added in Summer 2019.
TALOS ARCTICA: PNCC’S MAIN SAMPLE SCREENING AND INITIAL DATA COLLECTION PLATFORM
The TFS Talos Arctica is an automated high-throughput transmission electron microscope (TEM) platform focused on single-particle analysis. The constant-power, C-Twin lens delivers outstanding optical performance to allow optimal balance of contrast and resolution. The Arctica is an ideal instrument for screening cryo samples, for exploratory data collection, and for moderately advanced training. It is also a very capable high-resolution data collection instrument, and is capable of achieving single-particle reconstructions of < 3Å. This cryo-TEM is outfitted with a Gatan K2 Summit DED and a Ceta 16M CMOS camera.
TITAN KRIOS: PNCC’S HIGH-RESOLUTION DATA ACQUISITION PLATFORM
The TFS Titan Krios cryo-transmission electron microscope is a state-of-the-art 300kV instrument optimized for both high-resolution 3D imaging of proteins and macromolecular assemblies, and high-resolution cellular tomography. Its revolutionary design and stability permit a full range of semi-automated applications, including 2D electron crystallography, single-particle analysis, cryo-electron microscopy, and cellular tomography of frozen hydrated samples. The use of 300kV energy, addition of a third condenser lens, and increased optical stability make this the ideal instrument for collecting very large datasets at highest possible resolutions.