INSTRUMENTS

Major instrumentation available at PNCC include 1 FEI Talos Arctica and 4 FEI Titan Krios instruments for training, sample screening and high-resolution data acquisition. Gatan K3 Direct Electron Detectors and sample autoloaders are installed on all microscopes for optimal resolution and throughput. Two Titan Krios incorporates a FEI phase plate for high-contrast applications in both cryo-electron tomography and single particle imaging. In addition, PNCC maintains a wide array of equipment sample characterization and preparation using single particle and tomography methods.
Arctica Microscopes
Arctica
Science Applications
Screening (limited), Single Particle
Equipped with
Autoloader, K3 DED
Performance
2.2Å Apoferritin or better
Availability Calendar
Future Upgrade
Fringe Free (TBD 2021)
Data Collection Rate
2000-10000 (Counting, K3)
Krios-1 Microscope
Krios-1
Science Applications
Screening (limited), Single Particle, Tomography
Equipped with
Autoloader, Falcon 3 DED, K3 DED
Performance
2.2Å Apoferritin or better
Availability Calendar
Future Upgrade
Fringe Free (TBD 2021)
Data Collection Rate
2000-10000 (Counting, K3), 600-1400 (Counting, F3), 200-6000 (Linear, F3)
Krios-2 Microscope
Krios-2
Science Applications
Screening (limited), Single Particle, Tomography
Equipped with
Autoloader, Falcon 3 DED, K3 DED
Performance
2.2Å Apoferritin or better
Availability Calendar
Future Upgrade
Fringe Free, Falcon 4, BioContinuum, E-CFEG (TBD 2021)
Data Collection Rate
2000-10000 (Counting, K3), 600-1400 (Counting, F3), 200-6000 (Linear, F3)
Krios-3 Microscope
Krios-3
Science Applications
Screening (limited), Single Particle, Tomography
Equipped with
Autoloader, Falcon 3 DED, Phase Plate, Bioquantum K3
Performance
2.2Å Apoferritin or better
Availability Calendar
Future Upgrade
Fringe Free (TBD 2021)
Data Collection Rate
2000-10000 (Counting, K3), 600-1400 (Counting, F3), 200-6000 (Linear, F3)
Krios-4 Microscope
Krios-4
Science Applications
Screening (limited), Single Particle, Tomography
Equipped with
Autoloader, Falcon 3 DED, Phase Plate, Bioquantum K3
Performance
2.2Å Apoferritin or better
Availability Calendar
Future Upgrade
Fringe Free (TBD 2021)
Data Collection Rate
2000-10000 (Counting, K3), 600-1400 (Counting, F3), 200-6000 (Linear, F3)