Major instrumentation available at PNCC includes 1 Thermo Scientific Talos Arctica and 4 Thermo Scientific Titan Krios instruments for training, sample screening and high-resolution data acquisition. Gatan K3 BioContinuum HD and Falcon 4i direct electron detectors are installed on all microscopes for optimal resolution and throughput. Two Titan Krios incorporate a Thermo Scientific phase plate for high-contrast applications in both single particle imaging and cryo-electron tomography. In addition, PNCC maintains a wide array of equipment sample characterization and preparation using single particle and tomography methods.

INSTRUMENTS | Pacific Northwest Center for Cryo-EM

Arctica Microscopes

Arctica

Science Applications
Screening
Single Particle
Equipped With
Autoloader
K3 DED
Performance
2Å Apoferritin
Data Collection Rate (per 24 hrs)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-1 Microscope

Krios-1

Science Applications
Screening
Single Particle
Tomography
Equipped With
Autoloader
Falcon 4i DED
K3 DED
Fringe Free
Performance
1.6Å Apoferritin
Future Upgrade
Selectris X (TBD 2024)
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-2 Microscope

Krios-2

Science Applications
Screening
Single Particle
Tomography
Equipped With
Autoloader
Falcon 4i DED
K3 DED
BioContinuum K3
Fringe Free
CFEG
Performance
1.6Å Apoferritin
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-3 Microscope

Krios-3

Science Applications
Screening
Single Particle
Tomography
Equipped With
Autoloader
Falcon 3 DED
Phase Plate
BioContinuum K3
Fringe Free
Performance
1.6Å Apoferritin
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)
Krios-4 Microscope

Krios-4

Science Applications
Screening
Single Particle
Tomography
Equipped With
Autoloader
Falcon 3 DED
Phase Plate
BioContinuum K3
Fringe Free
Performance
1.6Å Apoferritin
Data Collection Rate (per 24 hrs)
200-6000 (Linear, F3)
600-1400 (Counting, F3)
2000-10000 (Super-res Counting, K3)
2000-18000 (Binned Counting K3)

Glacios 2 (TBD 2024)

Science Applications
Screening
Single Particle
Equipped With
Falcon 4i DED
Selectris