SerialEM Data Collection

A hands-on microscope operation for SerialEM SPA Data Collection. Prerequisite: SerialEM Introductory Training Workshop.

Dates: Spring 2024

Lodging: Room and Board provided

Accommodations: University Place Hotel

Location: RLSB

Who: PNCC Awarded General Access Proposal members only

The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five day on-site training workshop for 4-6 trainees on SerialEM data collection at PNCC in Spring 2024. This course is focused on training users to independently operate the microscope and collect data for single particle studies. This is an advanced course where students are required to have basic understanding of SerialEM and Microscope IU. Students are required to take the prerequisite course SerialEM Screening workshop prior to applying.

APPLY HERE


NOTE: Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Applications will open in 2024. 

Agenda

Hands-On Sessions, Group size 3 (max) per instrument, Each user should get 4-6 hours of time practicing
From 9am to 5pm (Pacific) each day:

Day 1

• Welcome & Workshop Overview
• Introduction to SerialEM Data Collection
• Demonstration of Workflow

Day 2

• Optimized Strategies for Data Collection
• Hands-on Microscope Instruction

Day 3

• Advanced Setup and Configurations
• Gatan BioContinuum Energy Filter and K3 Direct Electron Detector Setup and Tuning
• Associated Demonstrations by PNCC Staff
• Hands-on Microscope Instruction

Day 4

• Preparation for Data Collection Workflow Evaluation
• Data Collection Workflow Review
• Hands-on Practice

Day 5

• Student Evaluation (One-on-One Observation)
• Workshop Wrap-up & Next Steps