We offer training in cryo-EM methods via group workshops and 1-on-1 cross-training of users associated with awarded projects. Our first group workshop was held in August 2019 with 12 external trainees and lasted 3 weeks. Example datasets generated during the workshop are available for download on our BENCHMARKING AND WORKSHOP DATA page. We plan to offer another group workshop in early 2020 and more details about the logistics and how to apply will be available later this year.
For both group workshops and 1-on-1 cross-training, our curriculum consists of the following modules:
Sample vitrification (Vitrobot and Leica GP2)
Grid clipping and evaluation, cassette loading, autoloader operation
Sample evaluation (screening for ice thickness, particle distribution, level of contamination)
Microscope Operation (novice and advanced user trainings available)
DED (Standalone and post energy filter)
Data Collection Strategy
Data Collection using Phase Plate
For those interested in online resources, we recommend the following links to our partners in the NIH sponsored curriculum development programs
Grant Jensen’s "Getting started in cryo-EM course"
Comprehensive video material covering theoretical and practical matters in cryo-EM
Peter Shen and Janet Iwasa’s "Cryo-EM 101"
A web-based introductory course to cryo-EM that is interactive and self-paced
Fred Sigworth’s “Principles of cryo-EM"
In-depth technical treatment of cryo-EM theory: image formation and processing
Yingjie Chen and Weng Jiang’s “CryoVR”
Developing modules for VR training on cryo-EM instrumentation