EPU Data Collection

A remote microscope operation workshop for EPU SPA Data Collection.  
Prerequisite: SerialEM Introductory Training Workshop, SerialEM Data Collection Workshop or equivalent.

remote

Remote

 

Clock

3 Days

User

6-8
Trainees

Sun

Morning
Practical

Afternoon

Afternoon
Practical

Afternoon

No Cost

Dates:  November 4-6th, 2025 

Registration: FREE

Who: PNCC Awarded General Access Proposal members only


The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a three-day remote training workshop for 6-8 trainees on EPU data collection at PNCC Nov. 4-6th, 2025. This course is focused on training users to independently operate the microscope and collect data for single particle studies. This is an advanced course where students are required to have basic understanding of the Microscope UI. Students are required to take the prerequisite course SerialEM Screening workshop, SerialEM Data Collection workshop, or equivalent prior to applying.


APPLY HERE


NOTE: Workshop dates will be determined when enough suitable applicants have applied. To maximize impact, PNCC prefers to train one lab member per group with the intention that member further trains others in their group. Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Application closes September 15th, 2025, at 10am (Pacific). 

 

Agenda

Each day includes a combination of presentations, demonstrations, and hands-on practice. Workshop runs from 9:30am to 4pm (Pacific). Participants will receive one-on-one guided support during their next data collection session following the workshop to be granted independent status.

Day 1:

  • 9:30 Welcome and workshop intro
  • 10:00 EPU data collection overview
  • 12:00 Lunch break
  • 1:00 EPU data collection walkthrough continued
  • 3:00 Parameter selection
  • 4:00 End of day

Day 2:

  • 9:30 Discussion and review
  • 10:00 Hands-on beam setup in breakout groups
  • 12:00 Lunch break
  • 1:00 Hands-on data collection setup in breakout groups
  • 3:00 Group debrief and discussion
  • 4:00 End of day

Day 3:

  • 9:30 Demo of Multrigrid EPU and Smart EPU
  • 12:00 Lunch break
  • 1:00 Open hands-on practice in breakout groups
  • 3:00 Workshop wrap-up
  • 4:00 End of day