Sample Preparation (Jan. 2020)

Length: 3 days

Dates: January 21-23rd 2020

Cost: At no cost

Lodging: Room and Board provided

Trainees: 4-6 Trainees

Who: PNCC Awarded Proposal members only

Pacific Northwest Center for CryoEM (PNCC) would like to offer a three-day hands-on training workshop for 4-6 trainees on “Introduction to basic theory and hands-on practice in preparing cryo-EM specimens for SPA”. This short course will be held at PNCC between January 21-23rd, 2020. Students do not require any pre-existing knowledge of cryo-EM specimen preparation. Previous experience with negative-stain sample preparation may be useful but is not required.

At the end of this module students will:

  1. Be able to successfully freeze one of three benchmark samples using one (or more) of the following PNCC devices: TFS Vitrobot Mk IV, Leica GP2
  2. Clip and prepare samples for loading into an Autoloader equipped system
  3. Safely manipulate frozen samples for storage and shipping
  4. Visually identify basic issues arising from sample preparation and propose fixes

Optionally, with another day, students may also:

  1. Practice using their own samples with feedback from PNCC trainers
  2. Practice using different grid substrates, such as Lacey Film, AuFoil, thin-carbon, and graphene-oxide.