Microscope Operation Workshop (Aug. 2019)

Length: 3 weeks

Dates: Aug. 12 - 30th 2019

Cost: $3000 course fee

Lodging: Room and Board provided

Trainees: 12 Trainees

Who: Open to all

Location: RLSB, 2730 SW Moody Ave, Portland, OR 97220

Accommodations: PSU Summer Housing


Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a three-week training workshop for 12 trainees on experimental aspects of cryo-EM tailored to beginners held at Pacific Northwest Center for Cryo-EM between August 12-30th, 2019. This workshop will consist of 15-day hands-on training in: Sample Vitrification, Microscope Operation, and Automated Data Collection. This workshop does not cover data processing. Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. There is a $3000 course fee that covers accommodations, breakfast, lunch, consumables, and training.

At the end of this module students will:

  1. Be able to successfully freeze one of three benchmark samples using one (or more) of the following PNCC devices: TFS Vitrobot Mk IV, Leica GP2
  2. Clip and prepare samples for loading into an Autoloader equipped system
  3. Safely manipulate frozen samples for storage and shipping
  4. Visually identify basic issues arising from sample preparation and propose fixes
  5. Setup and run data collection on EPU and/or SerialEM on an Arctica and/or Krios
  6. Independently run the microscope