Past Workshop

Data Processing Workshop (Jun. 2020)

Length: 4 days

Dates: March 9-11th 2020 (Postponed due to Covid-19) June 22-25th 2020 

Cost: At no cost

Lodging: University Hotel Remote

Trainees: 4-6 Trainees

Who: PNCC Awarded Proposal members only

Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a three-day hands-on training workshop for 10 trainees on introduction to Relion and cryoSPARC, and cisTEM held at PNCC between March 9-11 June 22-25th 2020 remotely over zoom. Students will gain experience processing test dataset or their own PNCC datasets using this state-of-the-art software. Students do not require any pre-existing knowledge of cryo-EM 3D reconstruction, but familiarity of terminal sessions and unix command lines is required.

(All General Access Proposals have a limit of 1 PNCC Compute user account. If you want a PNCC Compute account and are affiliated with an approved PNCC General Access Proposal, contact Lauren.HalesBeck@pnnl.gov.)


Day 1

Cryosparc

09-10:30: Cryosparc overview - presented by developers
(Zoom-LIVE)

10:30-13: Cryosparc hands-on tutorial on training dataset #1
(Zoom-LIVE)

13-14: Lunch

14-17: Q&A session,
training on dataset #2

Day 2

Relion 3.1

09-13: Relion hands-on tutorial on training dataset #1
(Zoom-LIVE)

13-14: Lunch

14-17: Q&A session,
training on dataset #2



 

Day 3

Relion 3.1

09-13: Relion hands-on tutorial on training dataset #1 continue 
(Zoom-LIVE)

13-14: Lunch

14-17: Q&A session,
training on dataset #2



 

Day 4

Advanced Methods

09-11: Masks
(Zoom-LIVE)

11-13: Cryosparc Live 
(Zoom-LIVE)