Data Processing Workshop

Length: 5 half-days

Dates: Dec 7-11th, 2020

Cost: no cost

Trainees: 5-7 Trainees

Who: PNCC Compute Account (Cascade) Holders only

Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five half-day remote training workshop for 5-7 trainees on introduction to Relion and cryoSPARC over Zoom on Dec 7-11th, 2020. Students will gain experience processing test dataset or their own PNCC datasets using this state-of-the-art software. Students do not require any pre-existing knowledge of cryo-EM 3D reconstruction, but familiarity of terminal sessions and unix command lines is required.

--Application is now closed--

NOTE: Application to close November 16th, 2020 at 1pm PST. (All General Access Proposals have a limit of one PNCC Compute user account. If you want a PNCC Compute account and are affiliated with an approved PNCC General Access Proposal, contact Lauren.HalesBeck@pnnl.gov. Any new applications will be unable to attend this workshop due to time constraints.)


Remote Training

Day 1

CryoSPARC

09-11:
CryoSPARC overview - presented by CryoSPARC CEO and co-founder
(Pre-recorded session)

11-13:
CryoSPARC hands-on tutorial on training dataset #1
(Zoom-LIVE)

Day 2

CryoSPARC

09-13:
CryoSPARC training dataset on #1
(Zoom-LIVE)








 

Day 3

CryoSPARC/ Relion 3.1

09-11:
CryoSPARC training dataset on #2
(Zoom-LIVE)

11-13:
Relion hands-on tutorial on training dataset #1
(Zoom-LIVE)



 

Day 4

Relion 3.1

09-13:
Relion hands-on tutorial on training dataset #1 continue. 
Follow-up on cryoSPARC processing of dataset #2
(Zoom-LIVE)



 

Day 5

Advanced Methods

09-13:
Relion training on dataset #1 continue. 
Discuss results and wrap up. Masks/CryoSPARC live
(Zoom-LIVE)