Length: 5 days

Dates: TBD Oct. 2022, postponed to Early 2023

Cost: no cost

Trainees: 4-6 Trainees

Who: PNCC Awarded General Access Proposal members only

Location: RLSB, 2730 S.W. Moody Ave, Portland, OR 97201

Accommodations: University Place Hotel


The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five day on-site training workshop for 4-6 trainees on SerialEM data collection in Early 2023, date TBD. This course is focused on training users to independently operate the microscope and collect data for single particle studies. This is an advanced course where students are required to have basic understanding of SerialEM and Microscope IU. Students are required to take the prerequisite course SerialEM Introductory Training workshop prior to applying.

--Application not yet open--

NOTE: Applicant must be on an active General Access Proposal. PNCC prefers to train one lab member per group with the intention that this member can further train other members of their group. 

 


Agenda

Hands-On Sessions, Group size 3 (max) per instrument, Each user should get 4-6 hours of time practicing

Day 1

  • Welcome & Workshop Overview
  • Introduction to SerialEM Data Collection
  • Demonstration of Workflow

 
 

Day 2

  • Optimized Strategies for Data Collection
  • Hands-on Microscope Instruction

 


 

Day 3

  • Advanced Setup and Configurations
  • Gatan BioContinuum Energy Filter and K3 Direct Electron Detector Setup and Tuning
  • Associated Demonstrations by PNCC Staff
  • Hands-on Microscope Instruction

Day 4

  • Preparation for Data Collection Workflow Evaluation
  • Data Collection Workflow Review
  • Hands-on Practice

 
 

Day 5

  • Student Evaluation (One-on-One Observation)
  • Workshop Wrap-up & Next Steps