Microscope Operation Workshop (Aug. 2023)
Length: 2 weeks
Dates: 8/7-8/18
Cost: no cost
Lodging: Room and Board provided
Trainees: 4-6 Trainees
Who: Open to all
Location: RLSB, 2730 SW Moody Ave, Portland, OR 97220
Accommodations: University Place Hotel
Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a two-week training workshop for 4-6 trainees on experimental aspects of cryo-EM tailored to beginners held at PNCC from August 7th - 18th, 2023. This workshop will consist of 10-day hands-on training in: Sample Vitrification, Microscope Operation, and Automated Data Collection. This workshop does not cover data processing.
NOTE: Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Application closed June 26th, 2023 at 4pm.
Agenda:
Hands-On Sessions, Group size 3 (max) per instrument, Each user should get 4-6 hours of time practicing
Week 1
Day 1
- Introduction and Logistics
- Introduction to CryoEM Workflows
- Sample Screening Demonstration
Day 2
- Hands-on Supervised CryoEM Screening
- Sample Preparation and Collection Software Operation
Day 3
- Hands-on Supervised CryoEM Screening
- Sample Preparation and Collection Software Operation
Day 4
- Introduction to Data Collection
- TEM User Interface, Microscope Setup and Tuning, and Data Collection Software
Day 5
- Hands-on Supervised Data Collection
Week 2
Day 6
- Optimized Strategies for Sample Preparation and Data Collection
Day 7
- Hands-on Practice and Introduction to cryoSPARC Live
Day 8
- Final Evaluations: Sample Preparation & Microscope Operation
Day 9
- Final Evaluations: Sample Preparation & Microscope Operation
Day 10
- Wrap-up and Next Steps