Microscope Operation Workshop

Length: 2 weeks

Dates: TBD June 2022

Cost: no cost

Lodging: Room and Board provided

Trainees: 4-6 Trainees

Who: Open to all

Location: RLSB, 2730 SW Moody Ave, Portland, OR 97220

Accommodations: University Place Hotel

 

Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a two-week training workshop for 6 trainees on experimental aspects of cryo-EM tailored to beginners held at Pacific Northwest Center for Cryo-EM between TBD June 2022. This workshop will consist of 10-day hands-on training in: Sample Vitrification, Microscope Operation, and Automated Data Collection. This workshop does not cover data processing. Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. 

 


Hands-On Session: 2 Weeks, Group size 3 (max) per instrument, Each user should get 4-6 hours of time practicing

Week 1

Day 1 08-13: Onsite Badging, Test Quizzes, and Introductions

Day 1 13-14: Lunch Seminar - Sample preparation and EM Basics by Steve Reichow

Day 1 14-18: Tour and introduction of equipment by PNCC staff

Day 2 08-13: Sample Preparation Demonstration by PNCC staff

Day 2 13-14: Lunch Seminar - Introduction into SerialEM/EPU by Craig Yoshioka

Day 2 14-18: Microscope Demonstration by PNCC Staff

Day 3 08-18: Supervised user sample preparation

Day 4 08-18: Introduction into microscope operation

Day 5 08-13: Supervised user microscope operation

Day 5 13-14: Lunch Seminar - TBD

Day 5 14-18: Supervised user microscope operation

Week 2

Day 1 08-18: Supervised user microscope operation

Day 2 08-18: Supervised user microscope operation

Day 3 08-13: Supervised user microscope operation

Day 3 13-14: Lunch Seminar - CryoSPARC Live by Drew Gingerich

Day 3 14-18: Supervised user microscope operation

Day 4 08-13: Final Evaluation, sample preparation

Day 4 14-18: Final Evaluation, microscope operation

Day 5 08-13: Final Evaluation, sample preparation

Day 5 14-18: Final Evaluation, microscope operation