Length: 2 weeks

Dates: TBD 2023

Cost: no cost

Lodging: Room and Board provided

Trainees: 4-6 Trainees

Who: Open to all

Location: RLSB, 2730 SW Moody Ave, Portland, OR 97220

Accommodations: University Place Hotel


Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a two-week training workshop for 4-6 trainees on experimental aspects of cryo-EM tailored to beginners held at PNCC on TBD Summer 2023. This workshop will consist of 10-day hands-on training in: Sample Vitrification, Microscope Operation, and Automated Data Collection. This workshop does not cover data processing.

--Application not yet opened--

NOTE: Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology.

 


Agenda:

Hands-On Sessions, Group size 3 (max) per instrument, Each user should get 4-6 hours of time practicing

Week 1

Day 1

  • Introduction and Logistics
  • Introduction to CryoEM Workflows
  • Sample Screening Demonstration

Day 2

  • Hands-on Supervised CryoEM Screening
  • Sample Preparation and Collection Software Operation

Day 3

  • Hands-on Supervised CryoEM Screening
  • Sample Preparation and Collection Software Operation

Day 4

  • Introduction to Data Collection
  • TEM User Interface, Microscope Setup and Tuning, and Data Collection Software

Day 5

  • Hands-on Supervised Data Collection

Week 2

Day 6

  • Optimized Strategies for Sample Preparation and Data Collection

Day 7

  • Hands-on Practice and Introduction to cryoSPARC Live

Day 8

  • Final Evaluations: Sample Preparation & Microscope Operation

Day 9

  • Final Evaluations: Sample Preparation & Microscope Operation

Day 10

  • Wrap-up and Next Steps