This course is focused on training novice CryoEM users to independently vitrify samples and operate the microscope to collect data for single particle studies. Through hands-on practice, trainees will learn sample preparation, microscope operation, and automated data collection with SerialEM.
Dates: August 7-18, 2023
Lodging: Room and Board provided
Accommodations: University Place Hotel
Who: Open to the public
Pacific Northwest Center for Cryo-EM (PNCC) is offering a two-week training workshop, from August 7th to 18th, for 6 trainees on the complete CryoEM workflow. Upon completion, trainees will be able to vitrify liquid samples on autogrids, load the microscope, screen & collect data with SerialEM, and monitor their collection with cryoSPARC Live on-the-fly processing.
NOTE: Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Application closes June 26th, 2023 at 10am PDT.
From 9am to 5pm PT daily covering the following topics:
- Sample Preparation
- TFS Vitrobot
- Leica EM-GP2
- Manual Cryo-Plunger
- Autogrid Clipping
- Autoloader Cassette and Microscope Loading
- Microscope Tuning
- Energy Filter Tuning & K3 Gain Collection in Digital Micrograph
- SerialEM Sample Screening and Data Collection
- CryoSPARC Live on-the-fly processing
Hands-on practice sessions daily, maximum 3 trainees per instrument.
Each trainee will have the opportunity to earn CryoEM Merit badges.