EM SCHOOL

This course is focused on training novice CryoEM users to independently vitrify samples and operate the microscope to collect data for single particle studies. Through hands-on practice, trainees will learn sample preparation, microscope operation, and automated data collection with SerialEM.

Dates: TBD

Lodging: Room and Board provided

Accommodations: University Place Hotel or Hyatt House

Location: RLSB

Who: Open to the public

Pacific Northwest Center for Cryo-EM (PNCC) is offering a two-week training workshop in summer 2025, for 6 trainees on the complete CryoEM workflow. Upon completion, trainees will be able to vitrify liquid samples on autogrids, load the microscope, screen & collect data with SerialEM, and monitor their collection with cryoSPARC Live on-the-fly processing.

Application is currently closed

NOTE: Application will close three months prior to workshop start date 10am (Pacific). Preference of trainees is given to individuals who have research projects ready for Cryo-EM investigations and have background in structural biology.

AGENDA

From 9am to 5pm PT daily covering the following topics:

• Sample Preparation
  • TFS Vitrobot
  • Leica EM-GP2
  • Manual Cryo-Plunger
• Autogrid Clipping
• Autoloader Cassette and Microscope Loading
• Microscope Tuning
• Energy Filter Tuning & K3 Gain Collection in Digital Micrograph
• SerialEM Sample Screening and Data Collection
• CryoSPARC Live on-the-fly processing

Hands-on practice sessions daily, maximum 3 trainees per instrument.

Each trainee will have the opportunity to earn CryoEM Merit badges.