EM SCHOOL

A hands-on sample preparation, microscope operation, daily alignments, user interface, SerialEM and EPU training for automated data collection. This course is focused training users to independently operate the microscope and collect data for single particle studies.

On-site

Clock

2 weeks

User

4-6
Trainees

Sun

Daily Practical

Lunch

Lunch Lectures

no cost

No Cost

Dates: TBD Summer 2023

Lodging: Room and Board provided

Accommodations: University Place Hotel

Location: RLSB

Who: Open to the public


Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a two-week training workshop for 4-6 trainees on experimental aspects of cryo-EM tailored to beginners held at PNCC on TBD Summer 2023. This workshop will consist of 10-day hands-on training in: Sample Vitrification, Microscope Operation, and Automated Data Collection. This workshop does not cover data processing.


--Application Not Open--


NOTE: Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology.

 

Agenda

Hands-On Sessions, Group size 3 (max) per instrument, Each user should get 4-6 hours of time practicing
From 9am to 5pm (Pacific) each day:

Week 1

Day 1

  • Introduction and Logistics
  • Introduction to CryoEM Workflows
  • Sample Screening Demonstration

Day 2

  • Hands-on Supervised CryoEM Screening
  • Sample Preparation and Collection Software Operation

Day 3

  • Hands-on Supervised CryoEM Screening
  • Sample Preparation and Collection Software Operation

Day 4

  • Introduction to Data Collection
  • TEM User Interface, Microscope Setup and Tuning, and Data Collection Software

Day 5

  • Hands-on Supervised Data Collection

Week 2

Day 6

  • Optimized Strategies for Sample Preparation and Data Collection

Day 7

  • Hands-on Practice and Introduction to cryoSPARC Live

Day 8

  • Final Evaluations: Sample Preparation & Microscope Operation

Day 9

  • Final Evaluations: Sample Preparation & Microscope Operation

Day 10

  • Wrap-up and Next Steps