Length: 5 half-days

Dates: TBD Sept. 2022

Cost: no cost

Trainees: 5-7 Trainees

Who: PNCC Compute Account (Boreal) Holders only


The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five half-day remote training workshop for 5-7 trainees on introduction to Relion and cryoSPARC over Zoom on TBD September 2022. Students will gain experience processing test dataset or their own PNCC datasets using this state-of-the-art software. Students do not require any pre-existing knowledge of cryo-EM 3D reconstruction, but familiarity of terminal sessions and unix command lines is required.

Apply Now

NOTE: All General Access Proposals have a limit of one PNCC Compute user account. If you want a PNCC Compute account and are affiliated with an approved PNCC General Access Proposal, contact our User Office. Any new applications will be unable to attend this workshop due to time constraints. Workshops will be scheduled after obtaining enough applicants to fill a course, rolling application.

 


Agenda

From 9am to 1pm (Pacific) each day:

Day 1

CryoSPARC

09-11:
CryoSPARC overview - presented by CryoSPARC CEO and co-founder
(Pre-recorded session)

11-13:
CryoSPARC hands-on tutorial on training dataset #1
(Zoom-LIVE)

 

Day 2

CryoSPARC

09-13:
CryoSPARC training dataset on #1
(Zoom-LIVE)







 

Day 3

CryoSPARC/ Relion 3.1

09-11:
CryoSPARC training dataset on #2
(Zoom-LIVE)

11-13:
Relion hands-on tutorial on training dataset #1
(Zoom-LIVE)


 

Day 4

Relion 3.1

09-13:
Relion hands-on tutorial on training dataset #1 continue. 
Follow-up on cryoSPARC processing of dataset #2
(Zoom-LIVE)





 

Day 5

Advanced Methods

09-13:
Relion training on dataset #1 continue. 
Discuss results and wrap up. Masks/CryoSPARC live
(Zoom-LIVE)