SerialEM Data Collection

A hands-on microscope operation for SerialEM SPA Data Collection. Prerequisite: SerialEM Introductory Training Workshop or equivalent.

Dates:  Next workshop dates have not yet been determined

Travel Costs: Covered by applicant

Accommodations: Covered by applicant (discounts at local hotels are available through PNCC)

Location: RLSB

Who: PNCC Awarded General Access Proposal members only

The Pacific Northwest Center for Cryo-EM (PNCC) would like to offer a five-day on-site training workshop for 6-8 trainees on SerialEM data collection at PNCC from (dates TBD). This course is focused on training users to independently operate the microscope and collect data for single particle studies. This is an advanced course where students are required to have basic understanding of SerialEM and Microscope UI. Students are required to take the prerequisite course SerialEM Screening workshop prior to applying or have equivalent training.

APPLY HERE


NOTE: Workshop dates will be determined when enough suitable applicants have applied. To maximize impact, PNCC prefers to train one lab member per group with the intention that member further trains others in their group. Preference of trainees is given to individuals who have research projects ready for cryo-EM investigations and have background in structural biology. Application closes (TBD). 

Agenda

Each day includes a combination of presentations, demonstrations, and hands-on practice. Workshop runs from 9am to 5pm (Pacific) each day.

Day 1

• Welcome & Workshop Overview
• Introduction to the SerialEM Data Collection Workflow
• Hands-On Learning: Setting Up the Beam

Day 2

• Introduction to the Gatan Imaging Filter BioContinuum
• Introduction to Gain Collection on the K3 Direct Electron Detector
• Hands-On Learning: Beam and Instrument Setup

Day 3

• Introduction to Montaging, Multishot, and Target Selection
• Introduction to Camera and Scripts
• Hands-On Learning: Full Workflow

Day 4

• Review of Workflow
• Introduction to Parameter Selection
• Preparation for Merit Badge Evaluation

Day 5

• SerialEM Data Collection Merit Badge Evaluation
• Introduction to On-The-Fly Processing
• Workshop Wrap-Up and Next Steps