Sample Shipment

Shipping Instructions:

Please ship your clipped* grids in autoloader grid boxes using a Dry Shipper (Extended Time Cryogenic Refrigerator):

  • Include in the dry shipper a FedEx label to return the dewar.
  • A copy of your Grid Submission Form.
    • Do NOT ship samples unlisted** in your proposal or approved extension requests.
    • For BSL-2 samples, please mark clearly so that we can place into our BSL-2 dewar.
      • We cannot ship BSL-2 samples back, they will be destroyed after imaging.
  • Ship (First Overnight): any day after Monday, making sure we can receive them by Friday.
    • Please email your tracking number to scheduling@pncc.online.
    • For International shipments, please include a copy of all export paperwork needed to ship your dewar back. Make sure to complete this paperwork before shipment pickup to ensure the smoothest delivery with customs.

Preparing a Dry Shipper

Shipping Address:

Att: Claudia López   
Oregon Health & Science University
LVMS Mail Code: CL-P2M
2730 S. Moody Ave
Portland OR 97201

Questions

Where do I send pre-frozen grids?

Ship your grids any day after Monday, but please make sure that we can receive them by Friday. Please include in the dry shipper a FedEx label to return the dewar and a copy of your grid submission form. Once you have the FedEx tracking number for this shipment please forward this by email so we can track it. As soon as we receive the shipment we will let you know. Please send the dry shipper to:

    Att: Claudia López
    Oregon Health & Science University
    LVMS Mail Code: CL-P2M
    2730 S Moody Ave
    Portland OR 97201

How many grids can I send?

Per our scheduling policies, we request that only 11 grids are shipped to be screened in an 8 hour screening session. Up to 4 grids can be loaded for a scheduled data collection reservation. 

*Will you clip my grids?

All proposals have up to four hours of scientific point of contact time for sample preparation (should be used in the same day). After the allocated four hours, users should send frozen grids or come on-site to receive training to prepare their own samples. Please be aware that if your scientific point of contact needs to clip grids; your session might be delayed. If your group does not have access to a clipping station at your institution, we can provide the information needed to purchase one from ThemoScientific. Please contact support for more information. 

Can I bring my samples on-site to be vitrified at PNCC?

Yes, only if the samples have been included in the proposal's sample table and are biohazard safety level-1. We currently cannot accept liquid biohazard safety level-2 samples. Users will have access to a Vitrobot and Leica GP2 for sample preparation. All proposals have up to four of scientific point of contact time for sample preparation or training on the equipment.

 

What biosafety levels are accepted?

PNCC can only work with samples that are categorized biohazard safety level (BSL) 1 and 2. If the sample can be infectious or toxic in humans, animals or plants (viruses, bacteria, toxins etc.), then the risk group (RG) must be established, licenses for shipping obtained, and safe operating procedures defined according to biohazard safety level. This includes samples containing any agent (DNA) that is obtained and derived from sources that could potentially harbor (latently) such agents (e.g. serum-derived or obtained from human cell lines). Additional guidance on risk groups can be found in Section II of the Center for Disease Control and prevention in Biosafety in Microbiological and Biomedical Laboratories 5th Edition and references therein.  The starting point for our review will be the approval from the Institutional Biosafety Committee (IBC) from the applicant's home institution and the protocols on which the approval is based. Please attach both to this proposal after the biosketches. If the final sample might be considered RG1 but preparative work involved steps at BSL2, include a copy of your home institution IBC approval indicating a down-grading of BSL during the purification to support that designation. Generally, center staff will oversee approval of RG1 agents, while acceptance of RG2 agents from US institutions will require involvement of Oregon Health Science Univeristy's biosafety officer/ IBC. This may impact project award timeframes. Shipping agents from outside the US often requires additional approval from the Center for Disease Control and/or US Department of Agriculture, and will be considered on an individual basis. We cannot receive BSL2 samples in liquid form at this time. Only pre-frozen BSL2 samples with IBC documentation can be handled.

**Can I send other samples than what is listed in my proposal?

This depends. All requested samples must be in the same scientific scope to be approved. During the General Access Extension, approved users can request additional samples be added to their proposal. If BSL-2 samples are added, we will require a copy of your Institutional IBC for approval. Please contact support in all other cases.

 

Can we send hard drives?

Yes, this must be approved by scheduling before shipping hard drives to our facility.